Association of a 13.6-Megadalton Plasmid in Pediococcus pentosaceus with Bacteriocin Activity.
نویسندگان
چکیده
Pediococcus pentosaceus FBB61 and L7230, isolated from cucumber fermentations, produced a bacteriocin, designated pediocin A, which had identical activity spectra against species of Pediococcus, Clostridium, Staphylococcus, and Streptococcus. Both strains possessed a 13.6-megadalton plasmid (pMD136). Plasmid curing experiments suggested that both bacteriocin immunity and production determinants were encoded by pMD136. Use of pediocin-producing strains in food fermentations is discussed.
منابع مشابه
Plasmid-Associated Bacteriocin Production and Sucrose Fermentation in Pediococcus acidilactici.
Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subsp. dextranicum. Partial characterization of pediocin PA-1 is described. The mo...
متن کاملPlasmid DNA in Strains of Pediococcus cerevisiae and Pediococcus pentosaceus.
Five parental strains of Pediococcus were examined for plasmid content. Each strain contained three to six resident plasmids, ranging in size from 4.5 to 39.5 megadaltons. A bacteriocin-like substance produced by Pediococcus cerevisiae FBB63 was tentatively linked to a 10.5-megadalton plasmid after being cured with novobiocin.
متن کاملAntibacterial Activity of Cell-Free Culture Supernatant of Bacteriocinogenic Pediococcus pentosaceus IO1 against Staphylococcus aureus Inoculated in Fruit Juices
Background: Staphylococcus aureus is a major food-borne pathogen worldwide and a frequent contaminant of fruit juices. This study aimed to evaluate the antibacterial activity of bacteriocin-containing Cell-Free Culture Supernatants (CFCS) of Pediococcus pentosaceus IO1 against S. aureus inoculated in fruit juices, as well as their impact on the juice sensory attributes. Methods: The orange and...
متن کاملCharacterization of Antimicrobial Activities of Pediococcus pentosaceus Vtcc-B-601
Article history: Received on: 10/04/2014 Revised on: 06/05/2014 Accepted on: 19/05/2014 Available online: 27/05/2014 In this study, Pediococcus pentosaceus VTCC-B-601 was investigated and characterized for bacteriocin production. The antimicrobial activities were produced strongly at the late exponential phase (5x10 CFU/ml), corresponding to the activity of cephalosporin (13.3μg/ml) against Sal...
متن کاملThe Effect of Pediococcus pentosaceus Bacteriocin on Listeria monocytogenes in Soft Cheese
─Among antimicrobial materials produced by Pediococcus pentosaceus, bacteriocin have gained the greatest interest in food preservation as a natural substance. A bacteriocin producing P. pentosaceus was isolated from vegetables and human stool in a previous study. Purification, activity measurement, and characteristics of the bacteriocin was also determined. The yield of the bacteriocin was 9.08...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 50 6 شماره
صفحات -
تاریخ انتشار 1985